fatp 1 in  (MedChemExpress)

Journal: Scientific Reports

Article Title: Cannabidiol improves muscular lipid profile by affecting the expression of fatty acid transporters and inhibiting de novo lipogenesis

doi: 10.1038/s41598-023-30872-w

Figure Lengend Snippet: Effects of a high-fat diet (HFD) and two-week cannabidiol (CBD) administration on the fatty acid transport proteins expression, de novo lipogenesis, elongation and desaturation processes in rat myocytes. ↑, increase; ↓, decrease; red arrow inside the circle indicates the effects of seven weeks of high-fat diet feeding; green arrow inside the circle indicates the effects of two weeks of CBD treatment in HFD-fed rats; long-chain fatty acids (LCFA); fatty acid transport protein 1 and 4 (FATP-1 and -4); cytoplasmic and membrane-associated fatty acid binding protein (FABPc, FABPpm); fatty acid translocase (CD36); palmitic acid (C16:0); palmitoleic acid (C16:1n-7); stearic acid (C18:0); oleic acid (C18:1n-9); arachidic acid (C20:0); behenic acid (C22:0); lignoceric acid (C24:0); ATP citrate lyase (ACL); acetyl-CoA carboxylase (ACC); fatty acid synthase (FAS); fatty acid desaturase 2 (FADS2); stearoyl-coenzyme A (SCD1); elongation of very long-chain fatty acids proteins 1,3,6 and 7 (ELOVL1,3,6 and 7); glycerol-3-phosphate acyltransferase (GPAT); lysophosphatidic acid (LPA); 1-acylglycerol-3-phosphate-O-acyltransferase (AGPAT); phosphatidic acid (PA); phosphatidic acid phosphatase (PAP); diacylglycerol (DAG); diglyceride acyltransferase (DGAT); triacylglycerol (TAG). Figure was created in BioRender by the authors.

Article Snippet: The next step was blocking membranes in the Tris-buffered saline with Tween-20 (TBST) and 5% non-fat dry milk or 5% BSA, subsequently, the membranes were incubated overnight at 4 °C with selected primary antibodies: CD36 (sc-7309, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FABPpm (ab180162, Abcam, Cambridge, United Kingdom), FATP-1 (sc-25541, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FATP-4 (sc-5834, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), SREBP1 (sc-367, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FAS (3180S, Cell Signalling Technology, Danvers, MA, USA), FADS1 (ab126706, Abcam, Cambridge, United Kingdom), FADS2 (ab232898, Abcam, Cambridge, United Kingdom), ELOVL1 (ab230634, Abcam, Cambridge, United Kingdom), ELOVL3 (sc-54878, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and ELOVL6 (sc-385127, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Expressing, Binding Assay

Journal: Scientific Reports

Article Title: Cannabidiol improves muscular lipid profile by affecting the expression of fatty acid transporters and inhibiting de novo lipogenesis

doi: 10.1038/s41598-023-30872-w

Figure Lengend Snippet: The total expression of proteins involved in fatty acid uptake: fatty acid translocase (CD36), fatty acid binding protein (FABPpm) and fatty acid transport protein 1 and 4 (FATP-1 and -4), in the white and red gastrocnemius muscle. The total expressions of the abovementioned proteins are presented as percentage differences compared to the control group which was set as 100%. The data are expressed as mean values ± SD, n = 6 in each group; a p < 0.05 indicates a significant difference: the control group vs. the examined group; b p < 0.05 indicates a significant difference: HFD vs. HFD + CBD.

Article Snippet: The next step was blocking membranes in the Tris-buffered saline with Tween-20 (TBST) and 5% non-fat dry milk or 5% BSA, subsequently, the membranes were incubated overnight at 4 °C with selected primary antibodies: CD36 (sc-7309, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FABPpm (ab180162, Abcam, Cambridge, United Kingdom), FATP-1 (sc-25541, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FATP-4 (sc-5834, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), SREBP1 (sc-367, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FAS (3180S, Cell Signalling Technology, Danvers, MA, USA), FADS1 (ab126706, Abcam, Cambridge, United Kingdom), FADS2 (ab232898, Abcam, Cambridge, United Kingdom), ELOVL1 (ab230634, Abcam, Cambridge, United Kingdom), ELOVL3 (sc-54878, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and ELOVL6 (sc-385127, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Expressing, Binding Assay